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555466 Sigma-Aldrich

Rho, GST-Fusion, Human, Recombinant, E. coli

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Properties

Related Categories Auxiliary Proteins, Biochemicals and Reagents, Other, Proteins and Derivatives
Quality Level   100
assay   ≥90% (SDS-PAGE)
form   liquid
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C
capacity   ≥400 mmol/mol binding capacity (GTPγS)
shipped in   wet ice

Description

General description

Recombinant, human Rho fused to GST and expressed in E. coli. A small GTPase of the Ras superfamily. Regulates stress fiber formation in response to growth factor stimulation. Useful as a positive control in Western analysis and as an affinity precipitation reagent using immobilized glutathione resin.

Recombinant, human Rho fused to GST and expressed in E. coli. A small GTPase of the Ras superfamily. Regulates stress fiber formation in response to growth factor stimulation. Useful as a positive control in immunoblotting analysis and as an affinity precipitation reagent using immobilized glutathione resin.

Packaging

25 μg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 20 mM HEPES, 5 mM MgCl₂, 40% glycerol, 0.05% NaN₃, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Ridley, A. 1996. Curr. Biol. 6, 1256.
Ridley, A. and Hall, A. 1992. Cell 70, 389.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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