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  • 567333 - SNAPtide Botulinum Toxin A Substrate, Fluorogenic - Calbiochem

567333 Sigma-Aldrich

SNAPtide Botulinum Toxin A Substrate, Fluorogenic - Calbiochem

SNAPtide Botulinum Toxin A Substrate, Fluorogenic, is a synthetic peptide that acts as a FRET substrate with the fluorophore o-aminobenzoic acid and the acceptor chromophore 2,4-dinitrophenol (DNP).



Quality Level   100
assay   ≥90% (HPLC)
form   lyophilized
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
fluorescence   λex ~326 nm
  λem ~423 nm
shipped in   ambient


General description

A synthetic peptide, fluorescence resonance energy transfer (FRET) substrate containing the N-terminally-linked fluorophore o-aminobenzoic acid (Abz) and the acceptor chromophore 2,4-dinitrophenol (DNP) linked to the C-terminus. The substrate is based on the native botulinum toxin type A cleavage site found in the synaptosome-associated protein SNAP-25. Cleavage of the substrate by botulinum toxin releases the fluorophore and full fluorescence is restored.


200 nmol in Plastic ampoule

Biochem/physiol Actions

Cell permeable: no

Primary Target
Substrate for the native botulinum toxin

Product does not compete with ATP.

Reversible: no


Toxicity: Standard Handling (A)

Physical form

Lyophilized from water.

Preparation Note

Prepare a 5 mM stock solution of this peptide in DMSO as follows: Add 40 µl of DMSO to a vial containing 200 nmol of peptide.


Following reconstitution, aliquot, cover with foil to protect from light, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Bigalke, H. and Shoer, L.F. 2000. In: Handbook of Experimental Pharmacology,145, Bacterial Protein Toxins (K. Aktories and I. Just, Eds.) pp. 407-443, Springer-Verlag, Berlin.
Schiavo, G., et al. 1993. J. Biol. Chem.268, 23784.

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