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574796 Sigma-Aldrich

TBE Buffer, 10X Powder Pack, ULTROL Grade - Calbiochem

Each pack provides an adequate amount of material to make 1 L of 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

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Properties

Related Categories Loading Buffer and Tracking Dyes, Molecular Biology, Nucleic Acid Electrophoresis More...
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
storage conditions   +15C to +30C
color   white
shipped in   ambient

Description

General description

A commonly used electrophoretic buffer for the separation of DNA fragments. Each packet provides an adequate amount of material to make 1 liter of a 10X concentrate of buffer that can be diluted further. When diluted the 1X solution contains 89 mM Tris base, 89 mM boric acid, and 2 mM EDTA, pH 8.1-8.5.

Each pack provides an adequate amount of material to make 1 L of 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

Packaging

2 pk in Glass bottle

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 months in the refrigerator (4°C). Discard the buffer solution if any growth appears.

Reconstitute each package in ~750-800 ml H₂O and mix until completely dissolved. Add enough H₂O to bring the final volume to 1 liter. The resulting solution is a 10X concentrate that can be further diluted to 1X as needed.

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