• USA Home
  • 616376 - TRAIL, Human, Recombinant, NSO cells

EMAIL THIS PAGE TO A FRIEND
616376 Sigma-Aldrich

TRAIL, Human, Recombinant, NSO cells

Synonym: Apo2L; TNF-Related Apoptosis-Inducing Ligand

Purchase

Properties

Related Categories Auxiliary Proteins, Biochemicals and Reagents, Other, Proteins and Derivatives
Quality Level   100
assay   ≥97% (SDS-PAGE)
form   lyophilized
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   = -70C
impurities   ≤1 EU/μg Endotoxin (EU/μg TRAIL)
shipped in   ambient

Description

General description

Recombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.

Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.

Packaging

10 μg in Plastic ampoule

Biochem/physiol Actions

ED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.

Reconstitution

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.

Other Notes

Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343.
Mariani, S.M., et al. 1997. J. Cell Biol.137, 221.
Pan, G., et al. 1997. Science277, 815.
Pan, G., et al. 1997. Science276, 111.
Sheridan, J.P., et al. 1997. Science277, 818.
Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?