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648313 Millipore

Tris, Hydrochloride, ULTROL Grade - CAS 1185-53-1 - Calbiochem

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.1.

Synonym: tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Trizma® hydrochloride, Tromethane hydrochloride

  • CAS Number 1185-53-1

  • Linear Formula NH2C(CH2OH)3 · HCl

  • Molecular Weight 157.60

  •  Beilstein/REAXYS Number 3675235

  •  MDL number MFCD00012590

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Properties

Quality Level   100
assay   ≥99% (titration)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +15C to +30C
color   white
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
solubility   water: 500 mg/mL
cation traces   heavy metals: ≤5 ppm
shipped in   ambient
storage temp.   15-25°C
SMILES string   Cl.NC(CO)(CO)CO
InChI   1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key   QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Absorbance (1.0 M, H2O, 260 nm):≤0.1.

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: <0.1 at 260 nm, 1.0 M, H2O). A 1.0 M solution in H2O has a pH range of 3.5-5.0 and a pKa of 8.3 at 20°C.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

1 kg in Glass bottle

250, 500 g in Glass bottle

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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