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  • 650211 - Trypsin, Excision Grade, Bovine Pancreas

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650211 Sigma-Aldrich

Trypsin, Excision Grade, Bovine Pancreas

  • CAS Number 9002-07-7

  •  EC Index Number 3.4.21.4

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
availability   not available in Canada
color   white
feature productdata solubility   Soluble in HCl (1 mM) or trifluoroacetic acid (0.01%).
form   solid
shipped in   ambient
specific activity activity   ≥80 units/mg protein
storage condition   OK to freeze
  desiccated
brand family   Calbiochem
purity   ≥90% by HPLC
impurities   Chymotrypsin: ≤0.05%
storage conditions   +2C to +8C

Description

General description

Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, analysis of protein structural domains and cleavage of fusion proteins.

Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, protein structural domains, and cleavage of fusion proteins.

Packaging

100 μg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Stock solutions are stable for up to 1 month at -20°C or for up to 1 week at 4°C.

Unit Definition

1 unit is the amount of enzyme that will convert 1 µmol of substrate per min at 37°C in 100 mM Tris pH 8.5

Warning

B

Safety & Documentation

Safety Information

RTECS 
YN5075000

Documents

Certificate of Analysis

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Protocols & Articles
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