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650211 Sigma-Aldrich

Trypsin, Excision Grade, Bovine Pancreas

Synonym: Trypsin, Excision Grade, Bovine Pancreas

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
assay   ≥90% (HPLC)
form   solid
specific activity activity   ≥80 units/mg protein
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
storage conditions   +2C to +8C
color   white
solubility   trifluoroacetic acid: 0.01%
  HCl: 1 mM
foreign activity   Chymotrypsin ≤0.05%
shipped in   ambient

Description

General description

Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, analysis of protein structural domains and cleavage of fusion proteins.

Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, protein structural domains, and cleavage of fusion proteins.

Packaging

100 μg in Glass bottle

Warning

Toxicity: Irritant (B)

Unit Definition

1 unit is the amount of enzyme that will convert 1 µmol of substrate per min at 37°C in 100 mM Tris pH 8.5

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Stock solutions are stable for up to 1 month at -20°C or for up to 1 week at 4°C.

Safety & Documentation

Safety Information

Protocols & Articles
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