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650275 Sigma-Aldrich

Trypsin, Iodination Grade, Human Pancreas

Synonym: Trypsin, Iodination Grade, Human Pancreas

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   200
assay   ≥95% (SDS-PAGE)
form   lyophilized solid
specific activity activity   ≥2.5 units/mg protein
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
storage conditions   -20C
solubility   HCl: 2 mM
  aqueous buffer, pH 2.5: soluble
foreign activity   Chymotrypsin, none detected (when assayed against N-Suc-Ala-Ala-Pro-Phe-pNA (573462))
shipped in   wet ice

Description

General description

Native trypsin from human pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues.

Packaging

50 μg in Plastic ampoule

Warning

Toxicity: Irritant (B)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of N-benzyl-L-arginine-pNA per min at 25°C, 200 mM Tris-HCl, pH 7.8, 20 mM CaCl₂.

Physical form

Salt-free.

Preparation Note

Prepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C).

Other Notes

Merck Index13, 9865.
Travis, J., and Roberts, R.C. 1969. Biochemistry 8, 2884.

Safety & Documentation

Safety Information

Protocols & Articles
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