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  • 662057 - Ubiquitin, GST-Fusion, Recombinant, E. coli

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662057 Sigma-Aldrich

Ubiquitin, GST-Fusion, Recombinant, E. coli

Synonym: GST-Ub

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
assay   ≥95% (SDS-PAGE)
form   liquid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   = -70C
shipped in   wet ice

Description

General description

Recombinant ubiquitin fused at the N-terminus to GST and expressed in E. coli. Useful for glutathione affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using GST antibodies. Facilitates the visualization of polyUb chains due to larger ladder intervals.

Recombinant ubiquitin fused at the N-terminus to GST and expressed in E. coli. Useful for ubiquitination of protein substrates and subsequent glutathione affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using GST antibodies. Facilitates the visualization of polyUb chains due to larger ladder intervals.

Packaging

1 mg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM HEPES, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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