• USA Home
  • 684274 - β1,2-Xylosidase, Xanthomonas sp.

EMAIL THIS PAGE TO A FRIEND
684274 Sigma-Aldrich

β1,2-Xylosidase, Xanthomonas sp.

Synonym: β-D-Xylanxylohydrolase, β1,2-Xylosidase, Xanthomonas sp.

Purchase

Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
form   liquid
specific activity   ≥10 units/mL
  ≥20 units/mg protein
mfr. no.   Calbiochem®
storage condition   do not freeze
storage conditions   +2C to +8C
foreign activity   β-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases, none detected
shipped in   wet ice

Description

General description

Native β1,2-Xylosidase from Xanthomonas species. Catalyses the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of a1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Native β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Note: 1 mU = 1 milliunit.

Packaging

500 miu in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Reconstitution

Following initial use, refrigerate (4°C).

Other Notes

Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol.50, 1401.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?