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69076 Sigma-Aldrich

pET-32 Ek/LIC Vector Kit - Novagen

Novagen′s pET-32 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein and His-Tag and S-Tag thioredoxin protein

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Properties

Related Categories Bacterial Expression Vectors, Cloning and Expression, Molecular Biology, Sigma Expression Vectors
Quality Level   100
mfr. no.   Novagen®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   = -70C

Description

General description

The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein, and His•Tag and STag coding sequences that are cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Packaging

20 reactions in Glass bottle

Components

•1 µgEk/LIC Vector

•10 µlEk/LIC Control Insert

•25 UT4 DNA Polymerase, LIC-qualified

•50 µl10X T4 DNA Polymerase Buffer

•100 µl100 mM DTT

•50 µl25 mM EDTA

•40 µl25 mM dATP

•1.5 mlNuclease-free Water

•22 × 50 µlNovaBlue GigaSingles Competent Cells

•0.2 mlBL21(DE3) Competent Cells

•0.2 mlBL21(DE3)pLysS Competent Cells

•5 × 2 mlSOC Medium

•10 µlTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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