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69671 Millipore

Thrombin, Restriction Grade

Synonym: Thrombin, Restriction Grade

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   400
mfr. no.   Novagen®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C
shipped in   wet ice

Description

General description

Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.

Packaging

50 u in Plastic ampoule

Components

•50 UThrombin

•1 ml10X Thrombin Cleavage Buffer

•2 ml1X Thrombin Dilution/Storage Buffer

•10 µgCleavage Control Protein

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Unit Definition

One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.

Preparation Note

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles
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