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70746 Millipore

Benzonase® Nuclease, Purity > 90%

Synonym: Endonuclease from Serratia marcescens

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   300
assay   >90% (SDS-PAGE)
mfr. no.   Novagen®
storage condition   OK to freeze
storage conditions   -20C
shipped in   wet ice

Description

General description

Benzonase

Packaging

10 ku in Glass bottle

2.5 ku in Plastic ampoule

Application

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Benzonase® Nuclease Q&A

What type of nucleic acids does Benzonase® Nuclease work on? Can I use it when isolating RNA?
Keywords: Chromatography, Degradations, Enzyme activity, PAGE, Purification, Reductions

BugBuster® Plus Lysonase™ Kit: Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become an indispensable tool for efficient extraction of active soluble proteins from E. coli without the need for sonication or any other method of mechanic...
Keywords: Centrifugation, PAGE, Protein extraction, Sonication

Use of Benzonase® Nuclease to Prevent Clumping of Stored Peripheral Blood Mononuclear Cells (PBMCs) During Vaccine Evaluation

Ex vivo cytokine and memory T cell responses to the 42-kDa fragment of Plasmodium falciparum merozoite surface protein-1 in vaccinated volunteers
Keywords: Cancer, Clinical, Cryopreservation, Diseases, Immunology, Infectious Diseases

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become a valuable tool for extraction of proteins from E. coli without the need for mechanical disruption. It is also known that supplementing BugBuster® (as...
Keywords: Centrifugation, PAGE, Protein extraction, Purification, SDS PAGE

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