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70746 Millipore

Benzonase® Nuclease, Purity > 90%

Synonym: Endonuclease from Serratia marcescens



Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   300
assay   >90% (SDS-PAGE)
mfr. no.   Novagen®
storage condition   OK to freeze
storage conditions   -20C
shipped in   wet ice


General description



10 ku in Glass bottle

2.5 ku in Plastic ampoule


Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.


Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Benzonase® Nuclease Q&A

What type of nucleic acids does Benzonase® Nuclease work on? Can I use it when isolating RNA?
Keywords: Chromatography, Degradations, Enzyme activity, PAGE, Purification, Reductions

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Use of Benzonase® Nuclease to Prevent Clumping of Stored Peripheral Blood Mononuclear Cells (PBMCs) During Vaccine Evaluation

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Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become a valuable tool for extraction of proteins from E. coli without the need for mechanical disruption. It is also known that supplementing BugBuster® (as...
Keywords: Centrifugation, PAGE, Protein extraction, Purification, SDS PAGE

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