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70976 Millipore

SpinPrep PCR Clean-up Kit

Rapid purification of PCR products for downstream procedures



mfr. no.   Novagen®
storage condition   OK to freeze
storage conditions   +15C to +30C


General description

Rapid purification of PCR products for downstream procedures

The SpinPrep PCR Clean-Up Kit is designedfor the rapid purification of DNA amplifiedin PCR. The 10-minute procedure involvesaddition of a binding buffer followed byadsorption of the DNA to a silica membrane ina spin column format. Following a wash step,the DNA is eluted in low-salt buffer. This kitremoves DNA polymerases, dNTPs, salts, and> 99% of primers so that they do not interferewith downstream applications such as cloning,sequencing, or labeling. PCR products from100 bp to > 12,000 bp can be cleaned up, withrecoveries of amplified DNA in the range of60-90% under standard conditions.

Column binding capacity:up to 6 µg
PCR volume:100 µl/rxn
Typical recovery:60–90%
Size range:100 bp to > 12,000 bp
Time required:< 10 min


100 reactions in Plastic ampoule


•82 mlSpinPrep Bind Buffer

•27 mlSpinPrep Wash Buffer

•10 mlSpinPrep Elute Buffer

•100SpinPrep Filters

•100Receiver Tubes

•100Eluate Receiver Tubes


Toxicity: Multiple Toxicity Values, refer to MSDS (O)

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