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71091 Sigma-Aldrich

Nova Taq Hot Start DNA Polymerase

Heat-activatable modified form of recombinant Taq DNA polymerase

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Properties

Quality Level   100
mfr. no.   Novagen®
storage condition   OK to freeze
storage conditions   -20C
shipped in   wet ice

Description

General description

SourceRecombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration5.0 U/µl
EndonucleaseNone detected
ExonucleaseNone detected
Amplification effiencyFunctional PCR
Storage–20°C

Heat-activatable modified form of recombinant Taq DNA polymerase

NovaTaq<TMSYMBOL></TMSYMBOL> Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature. The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products. The enzyme must be activated by heat treatment (710 min at 95°C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with the Novagen Perfectly Blunt<TMSYMBOL></TMSYMBOL>, AccepTor<TMSYMBOL></TMSYMBOL>, and LIC Vector Kits.

Packaging

1250, 250 u in Glass bottle

Application

NovaTaq Hot Start DNA Polymerase is a heat-activatable modified form of recombinant Taq DNA polymerase

Features and Benefits

• Higher PCR specificity and yield
• Improved low-copy target amplification
• Ambient temperature setup compatible with automation
• Target amplification of up to 5 kbp
• Ideal for quantitative and high-throughput PCR applications

Components

•250 U or 5 × 250 UNovaTaq Hot Start DNA Polymerase (5 U/µl)

•1.5 ml or 5 × 1.5 ml10X NovaTaq Hot Start Buffer•1.5 ml or 5 × 1.5 ml25 mM MgCl₂

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-³²P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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