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71127 Sigma-Aldrich

Nus•Tag Monoclonal Antibody

Novagen®

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Properties

Related Categories Alphabetical Index, Antibodies, NS-NZ, Primary Antibodies
shipped in   wet ice
isotype   IgG1
Quality Level   100
mfr. no.   Novagen®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Sensitive, specific detection of Nus•Tag fusion proteins

The Nus•Tag<TMSYMBOL></TMSYMBOL> Monoclonal Antibody is a mouse monoclonal antibody (IgG1) with high affinity for the 54.8-kDa NusA protein from E. coli. This purified antibody specifically detects fusion proteins containing the Nus•Tag sequence expressed with the pET-43.1 and pET-44 vector series. Expression of polypeptides fused with the 495-aa NusA (Nus•Tag) can markedly enhance the solubility of recombinant proteins expressed in E. coli (Harrison 2000, Davis 1998).
The 50-g package size provides enough purified antibody to perform 50 Western blots (10 cm x 10 cm).

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

50 μg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Other Notes

Harrison, R.G. 2000. inNovations11, 4.
Davis, G.D., et al. 1998. Biotechnol. Bioeng.65, 382.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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