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71205-M Millipore

Benzonase® Nuclease HC, Purity > 90%

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
assay   >90% (SDS-PAGE)
mfr. no.   Novagen®
storage condition   OK to freeze
storage conditions   -20C

Description

General description

Benzonase<SUP>®</SUP> Nuclease is a genetically engineered endonuclease from <i>Serratia marcescens</i>. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminate<WBR>d oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster<TMSYMBOL></TMSYMBOL> andPopCulture<TMSYMBOL></TMSYMBOL> Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and removenucleic acids from <i>E. coli</i> extracts.<br/><br/>The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg<sup>2+</sup> for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by > 150 mM monovalentcations, > 100 mM phosphate, > 100 mMammonium sulfate, or > 100 mM guanidine HCl.<br/><br/>Benzonase Nuclease is available inultrapure (> 99% by SDS-PAGE) and pure(> 90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 10<sup>6</sup> U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied as a 0.2 µm filtered solution in 50% glycerol. Store at -20°C.<br/><br/><B>Total endotoxin:</b>< 0.25 EU/1,000 units.<B>Purity:</b> > 90% by SDS-PAGE.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

BugBuster® Plus Lysonase™ Kit: Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become an indispensable tool for efficient extraction of active soluble proteins from E. coli without the need for sonication or any other method of mechanic...
Keywords: Centrifugation, PAGE, Protein extraction, Sonication

Use of Benzonase® Nuclease to Prevent Clumping of Stored Peripheral Blood Mononuclear Cells (PBMCs) During Vaccine Evaluation

Ex vivo cytokine and memory T cell responses to the 42-kDa fragment of Plasmodium falciparum merozoite surface protein-1 in vaccinated volunteers
Keywords: Cancer, Clinical, Cryopreservation, Diseases, Immunology, Infectious Diseases

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become a valuable tool for extraction of proteins from E. coli without the need for mechanical disruption. It is also known that supplementing BugBuster® (as...
Keywords: Centrifugation, PAGE, Protein extraction, Purification, SDS PAGE

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