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71206 Millipore

Benzonase® Nuclease HC, Purity > 99%

Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

Synonym: Endonuclease from Serratia marcescens

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
assay   >99% (SDS-PAGE)
mfr. no.   Novagen®
storage condition   OK to freeze
storage conditions   -20C
impurities   <0.25 EU/kU Total endotoxin
shipped in   wet ice

Description

General description

Benzonase

Packaging

25 ku in Glass bottle

Application

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Benzonase® Nuclease Q&A

What type of nucleic acids does Benzonase® Nuclease work on? Can I use it when isolating RNA?
Keywords: Chromatography, Degradations, Enzyme activity, PAGE, Purification, Reductions

BugBuster® Plus Lysonase™ Kit: Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become an indispensable tool for efficient extraction of active soluble proteins from E. coli without the need for sonication or any other method of mechanic...
Keywords: Centrifugation, PAGE, Protein extraction, Sonication

Use of Benzonase® Nuclease to Prevent Clumping of Stored Peripheral Blood Mononuclear Cells (PBMCs) During Vaccine Evaluation

Ex vivo cytokine and memory T cell responses to the 42-kDa fragment of Plasmodium falciparum merozoite surface protein-1 in vaccinated volunteers
Keywords: Cancer, Clinical, Cryopreservation, Diseases, Immunology, Infectious Diseases

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become a valuable tool for extraction of proteins from E. coli without the need for mechanical disruption. It is also known that supplementing BugBuster® (as...
Keywords: Centrifugation, PAGE, Protein extraction, Purification, SDS PAGE

Peer-Reviewed Papers
15

References

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