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71508-M Millipore

D-Tube Dialyzer Maxi, MWCO 3.5 kDa

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Properties

Quality Level   100
mfr. no.   Novagen®
storage condition   OK to freeze
storage conditions   +15C to +30C

Description

General description


D-Tube Dialyzers Mini, Midi, Maxi, and Mega
The D-Tube Dialyzers be used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels. The disposable, single-use tubes require no syringes, microcentrifuge, or laborious steps to manipulate small sample volumes. The sample is added and removed using a standard laboratory pipet. Available with molecular weight cutoffs (MWCO) from 3.5 to 14 kDa, the D-Tube Dialyzers are designed in four volume capacities: mini (10-250 µl), midi (50-800 micro;l), maxi (100-3000 µl), and mega (10ml, 15ml, and 20 ml). The membrane is ultra-clean, EDTA-treated regenerated cellulose, sulfur- and heavy metal-free.

The D-Tube Dialyzers Mini, Midi, and Maxi contain 10 D-Tube Dialyzers and one floating rack that can hold up to four D-Tube Dialyzers. The D-Tube Dialyzers Mega contain 10 or 50 tubes and one or 5 floating racks to hold one D-Tube Mega in the exchange buffer.

D-Tube Electroelution Accessory Kit
The D-Tube Electroelution Accessory Kit provides a support tray for the D-Tube Dialyzers (compatible with most commercially-available horizontal electrophoresis units) and optimized reagents for protein and nucleic acid precipitation following electroelution. The kit also contains an optimized buffer for the removal of SDS from extracted protein samples, making them compatible with MALDI-MS analysis.The combination of D-Tube Dialyzers and D-Tube Electroelution Accessory Kit provides a unique tool for extraction of any protein, protein-protein and protein-DNA complexes from non-denaturing and denaturing (SDS) polyacrylamide gels with 60% recovery yield in less than 2 hours. The procedure efficientlyrecovers proteins and simultaneously removes ampholytes from proteins run on 2D gels. Extracted proteins are compatible with most downstream applications such as MALDIMS, animal immunization for antibodyproduction, HPLC analysis, peptide mapping, and functional assays. In addition, D-Tube Dialyzers can be used for oligonucleotides, RNA and DNA extraction from both polyacrylamide and agarose gels. Efficientextraction (>90%) is achieved for 15 nt oligos and for up to 80 kbp DNA fragments.

Warning

Toxicity: Standard Handling (A)

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

D-Tube™ Dialyzers Provide High Protein Recovery

Dialysis is commonly used to separate sample components by size. Separation is achieved by selective diffusion across a porous, semi-permeable membrane, which allows permeation of small molecules (e....
Keywords: BCA assay, Dialysis, Diffusion, Metabolites, Protein assay, Separation, Solvents

D-Tube™ Dialyzers

Gently and easily dialyze protein or nucleic acid samples using D-Tube™ dialyzers. These devices feature dialysis membrane windows in a capped centrifuge tube format, enabling efficient buffer exchan...
Keywords: Detergents, Dialysis

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