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8.51025 Sigma-Aldrich

5-Carboxyfluorescein

Novabiochem®

Synonym: 5-Carboxyfluorescein, 5-FAM

  • CAS Number 76823-03-5

  • Empirical Formula (Hill Notation) C21H12O7

  • Molecular Weight 376.32

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Properties

mfr. no.   Novabiochem®
storage conditions   Store at +2°C to +8°C.

Description

General description

This high purity fluorescent dye (λex494 nm, λem518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment [1].

The product number for this product was previously 01-63-0112.

To obtain a certificate of analysis (CoA) of a lot that begins with the letter “A”, please select the option in the right hand menu “Request a COA for Lot#s starting with A”.

Application

5-Carboxyfluorescein Novabiochem®. CAS 76823-03-5, molar mass 376.32 g/mol.

Linkage

Replaces: 01-63-0112

Analysis Note

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)
For TLC-Systems see our General Catalog

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Articles

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