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AB9354 Sigma-Aldrich

Anti-Beta III Tubulin Antibody

Anti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB.

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Properties

Related Categories Antibodies, Primary Antibodies More...
trade name   Chemicon
brand family   Chemicon
format   Affinity Purified
control   Brain tissue, Neuronal culture
molecular weight   55 kDa
application(s)   Immunocytochemistry
  Immunohistochemistry
  Western Blotting
isotype   IgY
clone   Polyclonal Antibody
purification method   ImmunoAffinity Purified
epitope   (Similar to TuJ1 antigen)
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Chicken
species reactivity   Human, Mouse, Rat
NCBI accession no.   NM_006086.2
UniProt accession no.   Q13509
gene symbol   MC1R(4157)
  TUBB3(10381)
  BETA-4
  TUBB4
material size   500 µL
shipped in   wet ice
storage conditions   Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

Description

Background information

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulin‎ is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue.

Physical form

Affinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide.
Four different antibodies were combined to make this product

Application

Immunocytochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells.

Immunohistochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues.

Optimal working dilutions must be determined by the end user

Specificity

Recognizes Beta III Tubulin

Immunogen

Synthetic peptides from human/rat Beta III Tubulin.

Quality Assurance

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot:
1:1000 dilution of this lot detected TUBULIN BETA III on 10 μg of mouse brain lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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