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AB9983 Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody

clone, from rabbit

Synonym: Tyrosine 3-hydroxylase, tyrosine 3-monooxygenase, tyrosine hydroxylase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TS-TZ
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   rat, mouse, rat, mouse
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_000351.2
UniProt accession no.   P07101

Description

General description

Tyrosine Hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specificity

Cat. # AB9983 recognizes the C-terminus of Tyrosine Hydroxylase

Reactivity with other species has not been determined.

Immunogen

Epitope: C-terminus

Recombinant protein

Application

Anti-Tyrosine Hydroxylase Antibody detects level of Tyrosine Hydroxylase & has been published & validated for use in IH(P) & WB.

Immunohistochemistry: 1:100 working dilutions were used on rat and mouse brain (paraffin embedded tissue).

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Target description

~ 58 kDa

Physical form

Antigen Affinity Purified

Format: Purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Rat Brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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