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Anti-LC3-I/II Antibody

This Anti-LC3-I/II Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of LC3-I/II.

Synonym: Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Affinity Purified
molecular weight   ~17/14 kDa observed
application(s)   Western Blotting
  Immunocytochemistry
clone   Polyclonal Antibody
purification method   Antigen Affinity Purified
concentration   Please refer to lot specific datasheet.
host   Rabbit
species reactivity   Human
NCBI accession no.   NP_115903
UniProt accession no.   Q9H492
gene symbol   MAP1A(4130)
  MAP1LC3A(84557)
  LC3
  LC3A
  LC3I
  LC3II
  MAP1BLC3A
material size   100 µL
shipped in   wet ice
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

Microtubule-associated LC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes.

Physical form

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Application

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected LC3 in serum-starved, chloroquine-treated HeLa cells. A punctate distribution is seen, evidence of autophagy conditions within the cell.

Specificity

This antibody detects both the non-lipidated LC3-I (non-autophagic) and lipidated LC3-II (autophagic) under autophagy conditions.

Immunogen

Recombinant protein corresponding to Human LC3-I/II.

Species Reactivity Notes

Human. Predicted to react with Bovine, Mouse, Porcine, Rat, Xenopus, Zebrafish based on 100% sequence homology.

Quality Assurance

Evaluated by Western Blotting in serum-starved, chloroquine-treated HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LC3-I (non-lipidated) and LC3-II (lipidated) in 10 µg of serum-starved, chloroquine-treated HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Autophagy and Mitophagy in Tumor Cell Survival and Death

Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle th...
Chandra Mohan, Ph.D.
MilliporeSigma, Temecula, CA
Keywords: Apoptosis, Autophagy, Bacterial conjugations, Cancer, Cell proliferation, Degradations, Diseases, Immunofluorescence, Oxidations, Phosphorylations, Reductions, Separation, Ubiquitinations, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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