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ABE52 Sigma-Aldrich

Anti-Cyclin D Antibody

Use Anti-Cyclin D Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP to detect Cyclin D also known as B-cell CLL/lymphoma 1, BCL-1 oncogene.

Synonym: B-cell CLL/lymphoma 1, BCL-1 oncogene, G1/S-specific cyclin D1, PRAD1 oncogene, cyclin D1, cyclin D1 (PRAD1: parathyroid adenomatosis 1)

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (ABE52)
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   rabbit
clone   polyclonal
purified by   affinity chromatography
shipped in   wet ice
species reactivity   primate, human, mouse
gene symbol   CCND1(595)
Species Reactivity Notes   Demonstrated to react with human and mouse. Predicted to react with primate based on 100% sequence homology.
gene symbol   BCL1
  D11S287E
  PRAD1
  U21B31

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~ 34 kDa

Analysis Note

Control
3T3/A31 cell lysate

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blot (SNAP ID) Analysis: 5 µg/ml from a previous lot detected Cyclin D on 10 µg of 3T3/A31 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cyclin D in NIH/3T3, A431, C2C12, and HeLa cells.

General description

Progression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human Cyclin D at the C-terminus.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in 3T3/A31 cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Cyclin D on 10 µg of 3T3/A31 cell lysate.

Specificity

This antibody recognizes Cyclin D at the C-terminus.

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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