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ABN78 Sigma-Aldrich

Anti-NeuN Antibody (rabbit)

Anti-NeuN (rabbit) Antibody, Cat. No. ABN78, is a highly specific rabbit polyclonal antibody that targets NeuN and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Synonym: Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN, Neuronal nuclei, RNA binding protein fox-1 homolog 3

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Affinity Purified
control   Mouse brain nuclear extract
molecular weight   ~48/42 kDa observed. 33.87/35.12 kDa (human isoform 1/2), 40.61/39.05/35.64/34.07/35.63 kDa (mouse isoform 1/2/3/4/5), 40.59kDa (rat; UniProt D4A2H6), 34.05 kDa (rat; UniProt A0A0G2K2J4) calculated. Uncharacterized bands may be observed in some lysate(s).
application(s)   Immunocytochemistry
  Immunohistochemistry
  Immunofluorescence
  Western Blotting
clone   Polyclonal Antibody
purification method   Affinity Purfied
epitope   N-terminus
concentration   Please refer to lot specific datasheet.
host   Rabbit
species reactivity   Human, Mouse, Rat, Snail
NCBI accession no.   NP_001034256
UniProt accession no.   Q8BIF2
gene symbol   RBFOX3(146713)
  D11BWG0517E
  HRNBP3
material size   100 µg
shipped in   wet ice
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

RNA binding protein fox-1 homolog 3 (UniProt Q8BIF2; also known as Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN, Neuronal nuclei) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID 52897) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Originally characterized as a marker of post-mitotic neurons and named neuronal nuclei (NeuN), Rbfox3 plays a role in promoting neuronal differentiation through alternative splicing of Numb pre-mRNA during brain development. Rbfox3-knockout in mice is reported to result in defective hippocampal gene expression, defects in synaptic transmission and plasticity in the dentate gyrus, as well as increased seizure susceptibility and decreased anxiety-related behaviors. Likewise, human RBFOX3 gene mutations have been linked to neurodevelopmental delay, cognitive impairments, autistic features, and epilepsy.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in cultured embryonic E18 rat hippocampus neurons.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN-positive neurons in human (cerebellum and cerebral cortex) and mouse (hippocampus) brain tissue sections.

Immunocytochemistry Analysis: A representative lots immunostained 4% paraformaldehyde-fixed neurons isolated from snail brain (Nikolić, L., et al. (2013). J. Exp. Biol. 216(Pt 18):3531-3541).

Immunofluorescence Analysis: Representative lots detected NeuN-positive neurons in frozen brain sections from 4% paraformaldehyde-perfused mice (Cherry, J.D., et al. (2015). J. Neuroinflammation. 12:203; Huang, B., et al. (2015). Neuron. 85(6):1212-1226; Ataka, K., et al. (2013). PLoS One. 8(11):e81744).

Immunofluorescence Analysis: A representative lots detected NeuN-positive neurons in formalin-fixed and paraffin-embedded amyotrophic lateral sclerosis (ALS) human brain tissue sections (Fratta, P., et al. (2015). Neurobiol. Aging. 36(1):546.e1-7).

Immunohistochemistry Analysis: A representative lot detected NeuN-positive neurons in rat brain tissue sections (Mendonça, M.C., et al. (2013).Toxins. 5(12):2572-2588).

Western Blotting Analysis: Representative lot detected NeuN in mouse and rat brain tissue lysates (Huang, B., et al. (2015). Neuron. 85(6):1212-1226; Mendonça, M.C., et al. (2013).Toxins. 5(12):2572-2588).

This rabbit polyclonal antibody is also avaiable as Alexa Fluor® 488 (ABN78A4), biotin (ABN78B), and Cy3 (ABN78C3) conjugates for immunocytochemistry, immunofluorescence, immunohistochemistry applications.

Specificity

This rabbit polyclonal antibody targets NeuN N-terminal region that ends at the start of RNA recognition motif (RRM). Target region is present in all human, mouse, and rat spliced isoforms reported by UniProt (A6NFN3, Q8BIF2, D4A2H6, A0A0G2K2J4).

Immunogen

GST-tagged recombinant mouse NeuN N-terrminal fragment.

Species Reactivity Notes

Human, Mouse, Rat, Snail.

Quality Assurance

Evaluated by Western Blotting in mouse brain nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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