• USA Home
  • ABN78C3 - Anti-NeuN (rabbit) Antibody, Cy3 Conjugate

EMAIL THIS PAGE TO A FRIEND
ABN78C3 Sigma-Aldrich

Anti-NeuN (rabbit) Antibody, Cy3 Conjugate

Anti-NeuN (rabbit) Antibody, Cy3 Conjugate is an antibody against NeuN (rabbit) for use in IC.

Synonym: NEUronal Nuclei, Neuronal Nuclei

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
format   Cy3
control   Rat E18 cortex cells
molecular weight   The unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa. An uncharacterized band appears at ~62 kDa in some lysates.
application(s)   Immunocytochemistry
clone   Polyclonal Antibody
purification method   Affinity Purfied
epitope   N-terminus
host   Rabbit
species reactivity   Rat, Mouse
gene symbol   NEUN
material size   100 µL
shipped in   wet ice
storage conditions   Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Description

Background information

ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Physical form

Purified rabbit polyclonal conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Specificity

This antibody recognizes NeuN at the N-terminus.

Immunogen

GST-tagged recombinant protein corresponding to mouse NeuN.

Species Reactivity Notes

Demonstrated to react with Mouse and Rat. The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human.

Quality Assurance

Evaluated by Immunocytochemistry in rat E18 cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Making Headway
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?