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ABN90 Sigma-Aldrich

Anti-NeuN Antibody

Detect NeuN using this Anti-NeuN Antibody validated for use in Western Blotting, IHC(P), ICC.

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Serum
control   Mouse brain E16 tissue lysate
molecular weight   ~48 kDa observed
application(s)   Western Blotting
  Immunohistochemistry (Paraffin)
  Immunocytochemistry
clone   Polyclonal Antibody
purification method   Unpurified
epitope   N-terminus
host   Guinea Pig
species reactivity   Mouse, Rat
material size   100 µL
shipped in   wet ice
storage conditions   Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Description

Background information

ABN90 is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Physical form

Guinea pig polyclonal serum containing 0.05% sodium azide.

Application

Immunohistochemistry Analysis: A 1:3,000 dilution from a representative lot detected NeuN in normal mouse cerebellum and frontal cortex tissues.

Immunocytochemistry Analysis: A 1:8,000 dilution from a representative lot detected NeuN in Rat E18 cortex and hippocampus primary cultures.

Specificity

This antibody recognizes the N-terminus of NeuN.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Quality Assurance

Evaluated by Western Blot in mouse brain E16 tissue lysate.

Western Blot Analysis: A 1:4,000 dilution of this antibody detected NeuN in 10 µg of mouse brain E16 tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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