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ABN90P Sigma-Aldrich

Anti-NeuN purified Antibody

This Anti-NeuN purified Antibody is validated for use in Western Blotting, ICC, IHC(P) for the detection of NeuN purified.

Synonym: NEUronal Nuclei, clone A60, NeuN

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (ABN90P)
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
biological source   guinea pig
clone   polyclonal
purified by   affinity chromatography
shipped in   wet ice
species reactivity   rat, mouse, human
molecular weight   ~48 kDa observed
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
gene symbol   NeuN
Quality Assurance   Evaluated by Western Blot in mouse E16 brain tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected NeuN on 10 µg of mouse E16 brain tissue lysate.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~48 kDa observed

Analysis Note

Control
Mouse E16 brain tissue lysate

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NeuN in mouse frontal cortex tissue.

Immunocytochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected NeuN in rat E18 cortex cells.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

General description

ABN90P is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Immunogen

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

This antibody recognizes the N-terminus of NeuN.

Physical form

Antigen Affinity Purified

Purified guinea pig polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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