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ABN91 Sigma-Aldrich

Anti-NeuN Antibody

This Anti-NeuN Antibody is validated for use in IHC(P), Western Blotting, ICC for the detection of NeuN.

Synonym: RNA binding protein fox-1 homolog 3, Fox-1 homolog C

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (ABN910-100UG)
  100 μg (ABN910-100UG)
  25 μg (ABN910-25UG)
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
biological source   chicken
clone   polyclonal
isotype   IgY
purified by   affinity chromatography
shipped in   wet ice
species reactivity   mouse, rat
molecular weight   ~45 kDa observed. Uncharacterized bands appear at ~40 and ~50 kDa in some lysates.
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
Species Reactivity Notes   Demonstrated to react with Mouse and Rat. Other homologies: Human (94% sequence homology).
gene symbol   NeuN
Quality Assurance   Evaluated by Western Blot in mouse brain E16 tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NeuN on 10 µg of mouse brain E16 tissue lysate.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~45 kDa observed. Uncharacterized bands appear at ~40 and ~50 kDa in some lysates.

Analysis Note

Control
Mouse brain E16 tissue lysate

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortex cells.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in mouse brain, mouse hippocampus, and mouse frontal cortex tissues.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

General description

NeuN (RNA binding protein fox-1 homolog 3; Fox-1 homolog C) is a RNA-binding protein found exclusively in the nuclei of neuronal cells. It is a member of the evolutionarily conserved Fox-1 family and is mainly involved in splicing of RNA.

Immunogen

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

This antibody recognizes the N-terminus of NeuN.

Physical form

Affinity Purfied

Purified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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