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CBL218 Sigma-Aldrich

Anti-Keratin K3/K76 Antibody, clone AE5

Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (CBL218-I)
application(s)   immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  western blot: suitable
biological source   mouse
clone   AE5, monoclonal
isotype   IgG1
shipped in   wet ice
species reactivity   bovine, human, mouse, rabbit
gene symbol   KRT3(3850)
trade name   Chemicon
brand family   Chemicon
format   Purified
molecular weight   64 kDa
clone   Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
gene symbol   CK-3
  CK3
  Cytokeratin-3
  K3
  Keratin-3

Description

Target description

64 kDa

Application

Immunohistochemistry on frozen sections: 1:50.

Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).

Western blotting: 1:500.

Optimal working dilutions must be determined by the end user.

Immunogen

Rabbit corneal epithelial keratin

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.

Physical form

Format: Purified

The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.

Storage and Stability

For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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