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ELI-004-H Sigma-Aldrich

IL-4 ELISPOT Antibody Pair, Human

IL-4 ELISPOT Antibody Pair, Human is a highly specific mouse monoclonal antibody & that targets IL-4 & has been tested in ELISA.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

biological source   mouse
clone   8D4-8, monoclonal
  MP4-25D2, monoclonal
species reactivity   human
application(s)   ELISA: suitable
shipped in   wet ice

Description

General description

Interleukin-4 (IL-4) is a pleiotropic lymphoid cell growth factor that is produced by activated T cells, mast cells, and basophils. This potent cytokine works by stimulating the growth and activation of certain B cells and T cells, as well as a variety of other hematopoietic cell types, and is important for the regulation and development of T helper subsets. Based upon current literature, IL-4 has also been shown to exhibit anti-tumor activity in experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses. The Millipore human IL-4 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-4 secreting cells at the single cell level.

Specificity

Both the capture and the detection antibodies included in this kit react with human interleukin -4 (IL-4).

Application

Research Category
Inflammation & Immunology

Packaging

Sufficient reagents for five, 96-well plates.

Components

• Human IL-4 Capture Antibody, clone 8D4-8. Quantity: 500 µg.

• Human IL-4 Detection Antibody, clone MP4-25D2. Quantity: 100 µg.

• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.

Target description

~15 kDa

Physical form

Format: Purified

The 500 µg purified IL-4 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and the 100 µg purified IL-4 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C for up to six months from the date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Elispot assays: State-of-the-art tools for functional analysis of cellular immunology

Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qual...
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