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FCMAB317PE Sigma-Aldrich

Milli-Mark™ Anti-NeuN-PE Antibody, clone A60

This Milli-Mark Anti-NeuN-PE Antibody, clone A60 is validated for use in FC for the detection of NeuN.

Synonym: A60, Neuna60, Neuron-Specific Nuclear Protein



Related Categories Antibodies, Primary Antibodies More...
trade name   Milli-Mark
format   Phycoerythrin
control   U251 cells
application(s)   Flow Cytometry
isotype   IgG1
clone   Monoclonal Antibody
purification method   Protein A Purfied
host   Mouse
species reactivity   Human
gene symbol   A58
material size   100 tests
shipped in   wet ice
storage conditions   Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.


Background information

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5.

Physical form

Purified mouse monoclonal IgG1 conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA


Antibody recognizes Neuronal Nuclei.


Purified cell nuclei from mouse brain.

Quality Assurance

Evaluated by flow cytometry using U251 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

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