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GF008 Sigma-Aldrich

Keratinocyte Growth Factor Protein, Recombinant human

The Keratinocyte Growth Factor protein (KGF protein or FGF-7 protein) is a potent mitogen for keratinocytes & epithelial cells.

Synonym: KGF, FGF-7

  •  eCl@ss 32160405

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Properties

biological source   human
application(s)   cell analysis: suitable (cell culture)
shipped in   dry ice
Quality Level   100
assay   >95% (SDS-PAGE and HPLC)
activity   ≥1 x 105 U/mg
mfr. no.   Chemicon®
impurities   <0.1 ng/μg Endotoxin (of KGF; 1EU/μg)
input   sample type epithelial cells
NCBI accession no.   NM_002009.2
UniProt accession no.   P21781
Gene Information   human ... FGF7(2252)

Description

General description

Keratinocyte Growth Factor (KGF or FGF-7) is a potent mitogen for keratinocytes and epithelial cells. Human KGF is a 18.9 kDa protein containing 163 amino acid residues.

Product Source: E.coli

Physical form

Lyophilized from 15 mM Sodium Phosphate pH 7.0 + 0.25 M NaCl.

Storage and Stability

The lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. After a quick spin, reconstitute in PBS to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted KGF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The ED50 was determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is <= 10 ng/mL

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
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