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  • GF051 - Granulocyte Colony-Stimulating Factor Protein, Recombinant human

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GF051 Sigma-Aldrich

Granulocyte Colony-Stimulating Factor Protein, Recombinant human

The Granulocyte Colony-Stimulating Factor protein (or G-CSF Protein), Recombinant human, is a potent stimulatory of bone marrow cells, especially those of neutrophil lineage.

Synonym: G-CSF

  •  eCl@ss 32160405

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Properties

Quality Level   100
biological source   human
assay   >98% (SDS-PAGE)
activity   ≥1x107 U/mg
mfr. no.   Chemicon®
application(s)   cell analysis: suitable (cell culture)
input   sample type hematopoietic stem cell(s)
NCBI accession no.   NM_000759.2
  NM_172219.1
  NM_172220.1
UniProt accession no.   P09919
shipped in   dry ice

Description

General description

Granulocyte Colony-Stimulating Factor (G-CSF) is a potent stimulatory of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Human G-CSF is an 18.7 kDa protein containing 174 amino acid residues.

Physical form

Lyophilized from 5 mM Sodium Citrate, pH4.0.

Storage and Stability

The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute with sterile water to a concentration of 0.1-0.5 mg/mL. This solution can be diluted into other buffered solutions. Reconstituted GF051 should be stored in working aliquots at -20°C for up to 6 months. Further dilutions should be made into buffer containing carrier protein or medium containing serum.

Analysis Note

Specific Activity: GF051 is fully biologically active when compared to standards.The ED(50) was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is <=0.1 ng/ml

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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