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GF109 Sigma-Aldrich

Ciliary Neurotrophic Factor Protein, Recombinant human

The human Ciliary Neurotrophic Factor (or CNTF protein) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro.

Synonym: CNTF

  •  eCl@ss 32160405

  •  NACRES NA.75

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Properties

biological source   human
shipped in   dry ice
Quality Level   100
assay   >98% (SDS-PAGE and HPLC)
mfr. no.   Chemicon®
impurities   <0.1 ng/μg Endotoxin (of CNTF; 1EU/ug)
NCBI accession no.   NM_000614.2
UniProt accession no.   P26441
Gene Information   human ... CNTF(1270)

Description

General description

Ciliary Neurotrophic Factor (CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. CNTF also has been shown to promote survivability and differentiation of other neural cell types. Recombinant Human CNTF is a 22.7 kDa protein containing 199 amino acid residues.

Product Source: E.coli

Physical form

The sterile filtered solution was lyophilized from 10 mM Citric Acid, pH 3.0 + 75 mM sodium chloride.

Storage and Stability

The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C. Reconstitute in 5 mM NaP, pH 7.5 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted human CNTF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150 ng/mL.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers
15

References

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