• USA Home
  • GF175 - WNT-1, Human Recombinant

EMAIL THIS PAGE TO A FRIEND
GF175 Sigma-Aldrich

WNT-1, Human Recombinant

Synonym: WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog

  •  eCl@ss 32160405

  •  NACRES NA.75

Purchase

Properties

Quality Level   100
assay   >98% (SDS-PAGE and HPLC)
application(s)   cell culture | stem cell: suitable
impurities   <0.1 μg/mg protein endotoxin (<1EU/μg)
UniProt accession no.   P04628
shipped in   dry ice

Description

General description

Product Source: E. coli.

Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.

Specificity

Cross Reactivty
Mouse

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Quality

Bioactivity assay: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

Physical form

Product is filtered through a 0.2 micron filter before lyophilization. Product is presented in .

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Wnt / Beta(β)-Catenin Signaling

Background Wnt Signaling Components    a) Wnt protein      b) Receptors of Wnt proteins    c) Wnt signaling modulators Wnt /beta-catenin signaling mechanism    a) Wnt signaling – Off    b) Wnt signal...
Keywords: Adhesion, Apoptosis, Cancer, Cell division, Cell proliferation, Clinical, Degradations, Gene expression, Hormones, Ligands, Metabolism, Phosphorylations, Transcription, Transduction, Ubiquitinations

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?