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GF220 Sigma-Aldrich

Neurite growth protein L1

The Neurite Growth Protein L1 is a cell adhesion molecule of the Ig superfamily (IgCAM) plays a critical role in the formation of neuronal networks.

  •  eCl@ss 32160405

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Properties

Quality Level   100
biological source   human
assay   >90% (Coomassie blue staining, SDS-PAGE)
mfr. no.   Chemicon®
concentration   1 mg/mL
shipped in   dry ice

Description

General description

L1, a cell adhesion molecule of the Ig superfamily (IgCAM) plays a critical role in the formation of neuronal networks. This is reflected by the variety of clinical signs associated with the X-linked recessive neurological disorder that is caused by mutations in the L1 gene. L1 regulates the formation of axon fascicles and promotes neurite outgrowth through interaction with a wide spectrum of binding partners including cell adhesion molecules and extra-cellular matrix components. L1 participates in the signaling of a secreted guidance cue of the Semaphorin family, Sema3A. Three types of experimental evidence support L1 as a key component of the Sema3A receptor complex.

Chimeric protein of Human neurite growth protein L1 and Human IgG-Fc γ domain purified using protein A-agarose (Millipore Corp, cat. # 16-125). MW ~ 200 kDa.

Application

Used at 5 μg/ml (see references 1 and 2).
Optimal working concentration must be determined by end user.

Physical form

100 µg of recombinant protein in 100µl of 1X PBS, 20%(v/v) glycerol. Frozen solution.

Analysis Note

Western blotting by primary antibody rat anti L1 (Millipore Corp, cat.# MAB5272) and secondary antibody goat anti rat, HRP (Millipore Corp, cat.# AP183P),Single band expected at ~ 200 kDa

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Safety Information

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