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GR11 Millipore

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)

liquid, clone αIR3, Calbiochem®

Synonym: Anti-IGF-R, Anti-Insulin-Like Growth Factor Receptor, Anti-Insulin-Like Growth Factor Receptor, Anti-IGF-R

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Properties

Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
clone   αIR3, monoclonal
biological source   mouse
species reactivity   human
form   liquid
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   do not freeze
storage conditions   +2C to +8C

Description

General description

Purified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.

Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.

This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of IGF-IR (Ab-1).

Immunogen

Human

partially purified, human placenta IGF-I receptor

Application

Immunoblotting (not recommended)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Neutralization Studies (1 µg/ml; use Cat. No. GR11L)

Paraffin Sections (not recommended)

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100 μg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Negative Control
HS27 cells

Positive Control
HepG2 cells

Other Notes

Anti-IGF-I Receptor (Ab-1) Mouse mAb (αIR3) immunoprecipitates the α and β subunits of the IGF-I receptor. Blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor. It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in indvidual systems.

Roth, R. 1988. Science239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm.149, 276.
Rosen, O.M., 1987. Science257, 1452.
Rechler, M.M. and Nissley, S.P., 1986. Hormone Res.24, 152.
Ullrich, A., et al. 1986. EMBO J.5, 2503.
Zapf, S. and Froesch, E.R., 1986. Hormone Res.24, 121.
Humbel, R.E., 1984. I. Chemistry; in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York).
Kull, F.C., et al. 1983. J. Biol. Chem.258, 6561.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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