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IF014 Sigma-Aldrich

Interferon-β Protein, Recombinant human

Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a).

Synonym: Fibroblast Interferon

  •  eCl@ss 32160405

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Properties

Quality Level   100
biological source   human
assay   ≥95% (one N-glycosylation site)
activity   3.99 x 108 U/mg
mfr. no.   Chemicon®
concentration   1 x 106 units/mL
application(s)   cell analysis: suitable (cell culture)
NCBI accession no.   NM_002176.2
UniProt accession no.   P01574
shipped in   dry ice

Description

General description

Product Source: cDNA obtained from human fibroblast mRNA expressed in CHO cells.

Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a). Molecular weight is ~23 kDa.

Linkage

Replaces: IF008

Physical form

Frozen liquid in 50 mM NaOAc, pH 5.5, containing 0.1% BSA.

Storage and Stability

After receipt, this product should be kept at -70°C or below for retention of full activity.
Thaw product vial by incubation cold tap water until just thawed. Thawed IFNβ should be
stored in working aliquots at -70°C for up to six months. Avoid multiple freeze/thaw cycles.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the
product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Analysis Note

Specific Activity: Determined by assaying inhibition of virus-induced cytopathic effect.In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%.The units are determined with respect to the international reference standard for human interferon beta (Hu-IFNbeta) provided by the National Institutes of Health.Unit of activity measured on VERO cells with vesicular stomatitis virus (VSV).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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