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IL006 Sigma-Aldrich

Interleukin-6 Protein, Recombinant human

The recombinant human Interleukin-6 protein (or IL-6 protein) is a potent lymphoid cell growth factor which affects B-lymphocytes, T-lymphocytes & hybridoma cells.

Synonym: IL-6

  •  eCl@ss 32160405

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Properties

Quality Level   100
biological source   human
assay   >98% (SDS-PAGE and HPLC)
activity   ≥1 x 10(E7) U/mg
mfr. no.   Chemicon®
application(s)   cell analysis: suitable (cell culture)
impurities   <0.1 ng/μg Endotoxin (of IFN gamma; 1EU/ug)
input   sample type hematopoietic stem cell(s)
NCBI accession no.   NM_000600.2
UniProt accession no.   P05231
shipped in   dry ice

Description

General description

Interleukin-6 (IL-6) is a potent lymphoid cell growth factor which affects B-lymphocytes, T-lymphocytes and hybridoma cells. It will also affect cytotoxic T-cells in combination with other factors such as IL-2 and g-interferon. Human IL-6 is a 20.9 kDa protein containing 184 amino acid residues.

Application

For most in vitro applications, IL-6 exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): B-cells, hybridoma cells.

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 7.5.

Storage and Stability

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 – 0.5 mg/mL. Gently shake the solution for about 10 minutes for better solubility. Do not vortex. The solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C or -80°C.

Analysis Note

Specific Activity: Human IL-6 is fully biologically active when compared to standards.The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent mouse 7TD1 cells is <= 0.1 ng/mL

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
WGK Germany 
WGK 1
Protocols & Articles
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