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IP04 Millipore

Protein G Plus-Agarose Suspension

Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, Molecular Biology, Protein A, G, Protein A, G and L Resins,
form   slurry (Liquid)
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   do not freeze
storage conditions   +2C to +8C
shipped in   wet ice

Description

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1.

Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

Packaging

1.5 ml in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Other Notes

Agarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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