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MAB1562 Sigma-Aldrich

Anti-Prion Protein Antibody, a.a. 109-112, clone 3F4

This Anti-Prion Protein Antibody, a.a. 109-112, clone 3F4 is validated for use in ELISA, IH, IH(P), IP, WB for the detection of Prion Protein.

Synonym: PrP, CD230

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Miscellaneous, Primary Antibodies More...
packaging   100 µg (MAB1562)
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   mouse
clone   3F4, monoclonal
  monoclonal
isotype   IgG2a
mfr. no.   Chemicon
shipped in   dry ice
species reactivity   hamster, human
gene symbol   GSS(2937)
  PRNP(5621)
  ASCR
  CD230
  CJD
  MGC26679
  PRIP
  PRP
  PrP27-30
  PrP33-35C
  PrP
  PrPc

Description

Target description

12.3 kDa

Application

Immunohistochemistry(paraffin):
Representative images from a previous lot. Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Brain

Immunohistochemistry (Kitamoto et al., 1987):
1:100-1:1,000 *See protocol below.

Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:
a. formic acid for 10 minutes at room temperature (Kitamoto et al., 1987)
b. hydrolytic autoclaving (Kitamoto et al., 1991)
c. microwaving (BioGenex, San Ramon, CA)

Western Blot: (Kascsak, R.J., 1991; Kascsak, R.J., 1987):
1:10,000-1:100,000 dilution of a previous lot was used.

Immunoprecipitation: (Kascsak, R.J., 1991; Kascsak, R.J., 1987):
1:10-1:100 dilution of a previous lot was used.

ELISA: (Kascsak, R.J., 1991; Kascsak, R.J., 1987):
1:100,000 dilution of a previous lot was used.

Optimal working dilutions must be determined by end user.

General description

Prions are thought to cause a number of diseases in a variety of mammals, including bovine spongiform encephalopathy (BSE, also known as "mad cow disease") in cattle and the Creutzfeldt-Jakob disease (CJD) in humans. All thus-far hypothesized prion diseases affect the structure of the brain or other neural tissue, and all are currently untreatable and thought to be fatal. Prions are hypothesized to infect and propagate by refolding abnormally into a structure which is able to convert normal molecules of the protein into the abnormally structured form. All known prions induce the formation of an amyloid fold, in which the protein polymerises into an aggregate consisting of tightly packed beta sheets. This altered structure is extremely stable and accumulates in infected tissue, causing cell death and tissue damage. This stability means that prions are resistant to denaturation by chemical and physical agents, making disposal and containment of these particles difficult.

Immunogen

Epitope: a.a. 109-112

Quality

Immunohistochemistry(paraffin):
Prion Protein (cat. # MAB1562) staining pattern/morphology in normal brain. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen predominantly as cell body staining of neurons.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Brain

Specificity

Prion protein, amino acid residues 109-112 of human, hamster and feline. Does not react with PrP from any other mammalian species. MAB1562 is reactive to native and denatured forms of PrP. Tissue or cells which have been fixed requires that the epitope be re-exposed (see below). Recognizes both protease sensitive and protease resistant forms of PrP.

Physical form

Format: Purified

Purified mouse monoclonal IgG2a in buffer containing PBS and no preservative.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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