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MAB1691 Sigma-Aldrich

Anti-Troponin I Antibody, a.a. 186-192, clone C5

Detect Troponin I using this Anti-Troponin I Antibody, clone C5 validated for use in ELISA & WB.

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Miscellaneous, Primary Antibodies More...
packaging   100 µg (MAB1691)
gene symbol   C5(727)
  TNNC1(7134)
  TNNI3(7137)
trade name   Chemicon
brand family   Chemicon
format   Purified
application(s)   ELISA
  Western Blotting
isotype   IgG2b
clone   Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Mouse
species reactivity   Bovine
  Chicken
  Fish
  Frog
  Human
  Mouse
  Rabbit
NCBI accession no.   NM_000363.4
UniProt accession no.   P19429
gene symbol   CMH7
  MGC116817
  cTnI
shipped in   1

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
POSITIVE CONTROL: heart or skeletal muscle.

Application

Immunohistochemistry. The antibody is not recommended for use on parafin sections.

Troponin sandwich ELISA (for labeling) with MAB3438.

Western blotting. Recognizes a band at approxinmately 22.5 kDa corresponding to troponin I.

Troponin I purification

Optimal working dilutions must be determined by the end user.

Research Category
Metabolism

Research Sub Category
Muscle Physiology

Immunogen

Bovine cardiac Troponin I.

Epitope: a.a. 186-192

Specificity

Troponin I. Reacts with Troponin I from heart and skeletal muscle.

Physical form

Purified immunoglobulin from Protein A Sepharose. Liquid in PBS pH 7.4 with 0.1% sodium azide as a preservative.

Storage and Stability

Maintain refrigerated at +2-8°C for up to 6 months.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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