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  • MAB2029 - Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27

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MAB2029 Sigma-Aldrich

Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27

Detect Chondroitin Sulfate Proteoglycan using this Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27 validated for use in FC, IF & IP.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CE-CH, Primary Antibodies
clone   9.2.27, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunofluorescence: suitable
  immunoprecipitation (IP): suitable
species reactivity   human
shipped in   wet ice
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_001897.4
UniProt accession no.   Q6UVK1

Description

Specificity

Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984). Reacts with human melanoma, glioma and proliferating brain endothelial cells.

Immunogen

Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose (Morgan et al., 1981).

Application

Immunofluorescence microscopy of cells in culture (Bumoi et al., 1984; Schrappe et al., 1992). Also, Legg J et al (2003) Development 130:6049-6063 {3% PFA fixed frozen sections}.

Immunoelectron microscopy (Bumoi et al., 1984)

Immunoprecipitation: 1μg/10E6 cells (Bumoi et al., 1984; Morgan et al., 1981)

FACS analysis: 1-2μg/10E6 cells

Functional block of melanoma cell spreading (Morgan et al., 1981), glioma cell proliferation3 and tumor growth in nude mice (Morgan et al., 1981; Schrappe et al., 1992).

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Physical form

Format: Purified

Purified immunoglobulin in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Storage and Stability

Maintain between 2 and 8°C in undiluted aliquots for up to 6 months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Chondroitinase for Neural Regeneration Research

The spinal cord is the information highway system for the brain. And axons are the road that allows information data to be transferred. So, when a traumatic spinal cord injury (SCI) occurs and axons ...
Keywords: Catalysis, Cell culture, Degradations, High performance liquid chromatography, Immunofluorescence, PAGE

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Peer-Reviewed Papers
15

References

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