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  • MAB3318 - Anti-TIMP-3 Antibody, a.a. 170-188, clone 136-13H4

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MAB3318 Sigma-Aldrich

Anti-TIMP-3 Antibody, a.a. 170-188, clone 136-13H4

Anti-TIMP-3 Antibody, a.a. 170-188, clone 136-13H4 is an antibody against TIMP-3 for use in IH(P) & WB.

Synonym: Tissue Inhibitor of Metalloproteinase-3

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (MAB3318)
availability   not available in
  not available in Japan
isotype   IgG1κ
gene symbol   TIMP3(7078)
trade name   Chemicon
brand family   Chemicon
format   Purified
molecular weight   26 kDa
application(s)   Immunohistochemistry (Paraffin)
  Western Blotting
clone   Monoclonal Antibody
  136-13H4
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Mouse
species reactivity   Human
  Rabbit
NCBI accession no.   NM_000362.4
UniProt accession no.   P35625
gene symbol   HSMRK222
  K222
  K222TA2
  SFD
  TIMP-3
shipped in   dry ice

Description

Disclaimer

Manufactured by Daiichi Fine Chemical Co., Ltd

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Breast carcinoma tissue

Application

Immunoblotting: 2 ug/mL

Immunohistochemistry (Paraffin):10ug/mL

Immunohistochemistry (Frozen): 2 ug/mL

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Immunogen

Epitope: a.a. 170-188

Oligopeptide of SWYRGWAPPDKSIINATDP (residue 170-188) on human tissue inhibitor of metalloproteinases-3.

Specificity

Specifically reacts with human TIMP-3. This is a purified mouse monoclonal antibody to an oligopeptide of SWYRGWAPPDKSIINATDP (residue 170-188) on human tissue inhibitor of metalloproteinases-3 (human TIMP-3).
MAB3318 cross-reacts with rabbit TIMP-3, but does not react with human TIMP-1 and human TIMP-2.

Physical form

Liquid in 0.1 M sodium phosphate buffer, pH 7.0 containing 2% protease free bovine Serum albumin.

Protein A Purfied

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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