• USA Home
  • MAB377A5 - Anti-NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate

EMAIL THIS PAGE TO A FRIEND
MAB377A5 Sigma-Aldrich

Anti-NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate

This Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate is validated for use in ICC, IHC for the detection of NeuN.

Synonym: NEUronal Nuclei, Neuronal Nuclei

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
format   AlexaFluor®555
control   Rat E18 primary cortex cells
molecular weight   The unconjugated parent antibody (Cat. No. MAB377) has an observed molecular weight in the 46-48 kDa range and possibly another band at approximately 66 kDa.
application(s)   Immunocytochemistry
  Immunohistochemistry
isotype   IgG1
clone   Monoclonal Antibody
  A60
purification method   Affinity Purfied
host   Mouse
species reactivity   Rat, Mouse
gene symbol   NEUN
material size   100 µL
shipped in   wet ice
storage conditions   Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Description

Background information

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

Physical form

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Application

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NeuN in mouse adult brain cryosections.

Specificity

NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia.

Immunogen

Purified cell nuclei from mouse brain.

Species Reactivity Notes

Demonstrated to react with Rat and Mouse. Unconjugated Anti-NeuN has been shown to work with multiple species including Human, Ferret, Chicken, and Avian.

Quality Assurance

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Making Headway
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?