• USA Home
  • MAB377X - Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated

EMAIL THIS PAGE TO A FRIEND
MAB377X Sigma-Aldrich

Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated

Anti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated is an antibody against NeuN for use in IH.

Synonym: Neuron-Specific Nuclear Protein

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   500 µg (MAB377X)
application(s)   immunohistochemistry: suitable
biological source   mouse
clone   A60, monoclonal
  monoclonal
isotype   IgG1
mfr. no.   Chemicon
shipped in   wet ice
species reactivity   mouse, rat, human
gene symbol   A60
  NeuN
conjugate   ALEXA FLUOR 488

Description

Disclaimer

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Brain tissue, most neuronal cell types throughout the adult nervous system

Application

Immunohistochemistry: 1:100 on rat (paraformaldehyde fixed) and mouse (paraformaldehyde fixed, antigen retrieved) brain tissue.



Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

General description

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

Immunogen

Purified cell nuclei from mouse brain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). MAB377X reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. The immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377X include Purkinje, mitral and photoreceptor cells.

Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones.

The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.

Physical form

Protein A Purfied

Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.

Storage and Stability

Maintain for 6 months at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Making Headway
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?