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MAB4133 Sigma-Aldrich

Anti-p16 Antibody, clone D25

Anti-p16 Antibody, clone D25 is a Mouse Monoclonal Antibody for detection of p16 also known as INK4a, MTS1, CDK4IN, CDKN2 & has been validated in WB & IHC.

Synonym: INK4a, MTS1, CDK4IN, CDKN2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (MAB4133)
application(s)   immunohistochemistry: suitable
  western blot: suitable
biological source   mouse
clone   D25, monoclonal
isotype   IgG1
shipped in   wet ice
species reactivity   human
gene symbol   CDKN2A(1029)
trade name   Chemicon
brand family   Chemicon
format   Purified
clone   Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
gene symbol   ARF
  CDK4I
  CDKN2
  CMM2
  INK4
  INK4a
  MLM
  MTS-1
  MTS1
  P16
  TP16
  p14
  p14ARF
  p16-INK4
  p16-INK4a
  p16
  p16INK4
  p16INK4A
  p16INK4a
  p19
  p19ARF

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
POSITIVE CONTROL: HeLa cells

colon carcinoma

Application

Immunoblotting

Immunohistochemistry

Optimal working dilutions must be determined by end user.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Immunogen

Recombinant p16 fusion protein from E. coli.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

P16* is a major tumor suppressor gene, which is known to be deleted or mutated in many types of human cancers. Antibody MAB4133 binds specifically to p16 in its recombinant or native form. It also recognizes p16 expressed by melanomas and neve in situ.

Physical form

Format: Purified

Liquid in phosphate buffer, containing azide as a preservative.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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