• USA Home
  • MAB8887 - Anti-Collagen Type II Antibody, clone 6B3

EMAIL THIS PAGE TO A FRIEND
MAB8887 Sigma-Aldrich

Anti-Collagen Type II Antibody, clone 6B3

Anti-Collagen Type II Antibody, clone 6B3 is an antibody against Collagen Type II for use in IF, IH & WB.

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   50 µg (MAB8887)
application(s)   immunofluorescence: suitable
  immunohistochemistry: suitable
  western blot: suitable
biological source   mouse
clone   6B3, monoclonal
  monoclonal
isotype   IgG1
mfr. no.   Chemicon
shipped in   wet ice
species reactivity   human, mouse, chicken, salamander
gene symbol   COL2A1(1280)
  ANFH
  AOM
  COL11A3
  MGC131516
  SEDC
  chondrocalcin

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

120 kDa

Analysis Note

Control
Cartilage in lung or fetus

Application

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blot (Mayne, 1994)

Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL, 30 minutes at room temperature. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37ºC.

Immunofluorescence

ELISA

Optimal working dilutions must be determined by end user.

Immunogen

Purified type II collagen; obtained from adult chicken sternum by limited pepsinization.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

In Western blotting (but not ELISA), MAB8887 recognizes both a1(II) and a3(XI) chains which have identical primary structure. In Western blotting, MAB8887 reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. It will not react with intact Collagen II under traditional conditions.

Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Immunoblotting of CNBr peptides of collagen II shows that MAB8887 reacts with CB11 (25kDa) fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule.

In pepsin solublized collagen II, MAB8887 reacts with a 95-97 kDa fragment, as well as, the native single chain of 120kDa. If propeptides are present, MAB8887 will detect a 200kDa fragment.

Physical form

Format: Purified

Protein G Purified

Purified immunoglobulin in 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.

Storage and Stability

Maintain at 2–8°C in undiluted aliquots for up to 12 months

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?