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MABE327 Sigma-Aldrich

Anti-p53 (pantropic) Antibody, clone DO-1

Use Anti-p53 (pantropic) Antibody, clone DO-1 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC, IF, IP to detect p53 (pantropic) also known as Cellular tumor antigen p53 or Antigen NY-CO-13.

Synonym: Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (MABE327)
application(s)   immunocytochemistry: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   mouse
clone   DO-1, monoclonal
isotype   IgG2aκ
shipped in   wet ice
species reactivity   human
gene symbol   TP53(7157)
format   Purified
molecular weight   ~53 kDa observed. Uniprot describes 9 different isoforms due to alternative splicing and post-translational modification. This antibody detects isoform 1 (~38 kDa), isoform 2 (~39 kDa), and isoform 3 (~39 kDa). An uncharacterized band may be observed at ~43 kDa in some cell lysates.
clone   Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
gene symbol   P53
Quality Assurance   Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected p53 (pantropic) in 10 µg of A431 cell lysate. This antibody detects isoforms 1, 2, and 3.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~53 kDa observed. Uniprot describes 9 different isoforms due to alternative splicing and post-translational modification. This antibody detects isoform 1 (~38 kDa), isoform 2 (~39 kDa), and isoform 3 (~39 kDa). An uncharacterized band may be observed at ~43 kDa in some cell lysates.

Analysis Note

Control
A431 cell lysate

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p53 (pantropic) in A431 cells.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal adenocarcinoma tissue and in human prostate cancer tissue.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal cancer cells.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in radiolabelled HOS cell lysate (Vojtesek, B., et al. (1995). 71(6):1253-1256.).

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in primary breast carcinoma tissue (Beck, T., et al. (1995). Gynecol Oncol. 57(1):96-104.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

General description

Cellular tumor antigen p53 (p53) is a ubiquitous transcription factor that is most widely known for its function as a tumor suppressor, through its regulation of cell growth and survival. p53 is activated in response to cell stress such as DNA damage and induces either cell cycle arrest or apoptosis. It coordinates the expression of multiple genes including p21, GADD45, Bax, and Puma. p53 is regulated by multiple mechanisms: At the post-transcriptional level, the stability of p53 mRNAs is regulated by the binding of molecules such as HuR, Wig-1, RPL26, L26, and WRAP53. Post-translationally, p53 undergoes phosphorylation and acetylation which may also play a role in regulating its activity. It may also undergo ubiquitination via the Akt-MDM2 pathway resulting in degradation by the 20S proteosome. Mutations in p53 are prevalent in many types of cancers, underscoring the therapeutic relevance of this protein.

Immunogen

Epitope: N-terminus

Recombinant protein corresponding to human p53 (pantropic).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

This antibody recognizes the N-terminus of p53 (pantropic).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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