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AB10554 Sigma-Aldrich

Anti-Tbr1 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: T-box, brain, 1, T-brain-1, T-box brain protein 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, T-TB
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable (paraffin)
species reactivity   opossum, bovine, rat, mouse, horse, mouse, pig, canine
species reactivity (predicted by homology)   canine (based on 100% sequence homology), rat (based on 100% sequence homology), equine (based on 100% sequence homology), porcine (based on 100% sequence homology), bovine (based on 100% sequence homology), opossum (based on 100% sequence homology)
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_033348
UniProt accession no.   Q64336
Gene Information   human ... TBR1(10716)

Description

General description

Tbr1 gene is a member of a conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Tbr1, a putative transcription factor known to be expressed in post-mitotic neurons, has been found highly expressed in glutamatergic early-born cortical neurons. A similar protein has been disrupted in mice and shown to be critical for early cortical development, and causes loss of projection neurons in the olfactory bulbs and olfactory cortex. The C-terminal region the Tbr1 derived protein was found to be necessary and sufficient for association with the guanylate kinase domain of calcium/calmodulin-dependent serine protein kinase.

Immunogen

Epitope: This antibody recognizes Tbr1 near the N-terminus.

KLH-conjugated linear peptide corresponding to mouse Tbr1 near the N-terminus.

Application

Anti-Tbr1 Antibody detects level of Tbr1 & has been published & validated for use in IH(P).

Immunohistochemistry Analysis: 1:400 dilution from a previous lot detected Tbr1 in mouse cerebellum tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Target description

74kDa calculated

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Immunohistochemistry in mouse frontal cortex tissue.

Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Tbr1 in mouse frontal cortex tissue.

Analysis Note

Control
Mouse frontal cortex tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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